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5) © 2009 by Taylor Francis Group, LLC CHAPTER 4 An Introduction to Fluorescence Microscopy: Basic Principles, Challenges, and Opportunities François Aguet, Ce ́dric Vonesch, Jean-Luc Vonesch, and Michael Unser The discovery of fluorescent labels suitable for in vivo use and the development of high-resolution microscopes have transformed the fluorescence microscope into a mainstream tool, bringing about a revolution for biological imaging and having a profound impact on the way research is being conducted in the life sciences.
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